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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Vol 11 No 2 (2006): June 2006" : 12 Documents clear
KEKAYAAN JENIS PORTUNIDAE DI SISI SHIPPING LINE SELAT MADURA Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/378

Abstract

A monitoring activity for the existence of portunid crabs in the narrowest part of Madura Strait has been done from 2001 to 2004. Crabs have been collected in June or July each year in day time during spring tide. Crab collection was carried out in four sampling station (I to IV) using 0,5 cm trawl net for 10 minutes netting in each time. The results show that during four year monitoring six species of portunid crab has been captured. Among the six species three of them, Charybdis affinis, C. anisodon, and Portunus pelagicus were collected each year even with difference level in local distribution. C. affinis and P. pelagicus have been monitored in the same three stations, and C. anisodon has also been monitored in three stations but differs from other three portunid crab species, C. annulata. P. trituberculatus, and Scylla paramamosain were very rare and have been captured in less then three sampling times collection. We conclude that in this area the portunid crab has been dominated by C. affinis which have less economic value, and for C. anisodon even the number higher than other portunid genus the occurrence is not stabil as in C. affinis.
KEBERADAAN TELUR CACING PARASIT PADA SISWA SD DI SEKITAR INSTALASI PENGOLAHAN AIR LIMBAH (IPAL) TERPADU KOTA MALANG DAN HUBUNGANNYA DENGAN KEPADATAN TELUR CACING PADA AIR LIMBAH PERUMAHAN DI IPAL TERPADU Sofia Ery Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/379

Abstract

The research to find out the existence, kinds, and prevalence of parasitic worm egg on the hands and nails of elementary school students lives in IPAL area also its existence in the water of housing dump has been conducted recently. It also analyze the correlation between the number of parasitic worm egg population on the elementary school students hands and nails lives in IPAL area and the water of housing dump in IPAL Terpadu Kota Malang. The research used descriptive explorative method and conducted on June–August 2004. The sample of parasitic worm egg is taken from elementary school students hands and nails ilves in IPAL area and the water of housing dump in IPAL Terpadu Kota Malang. The elementary shools taken as samples area SDN Tlogomas 1, SDN Bareng 3, SDN Mulyorejo 1, SDN Mergosono 1, and SDN Mergosono 2. The locations of IPAL Terpadu Kota Malang used in this research are Tlogomas, Bareng, Mulyorejo, Mergosono Gang 1, and Mergosono Gang 3 area. Identification of worm egg is conducted in Biology Departement, Faculty of Mathematics and Science, State University of Malang. The data taken are in the form of kinds of parasitic worm egg based on morphology characteristics and it will be analyzed descriptively. Then the prevalence of each kind of parasitic worm egg will bw counted. Then the researchers do some correlation analysis. Findings of this research show that from 5 samples of elementary school used in this research only the hands and nails of students in SDN Mulyorejo 1 have no human parasitic worm egg while in four others found 4 kinds of human parasitic Nematoda they are Ascaris lumbricoides, Enterobius vermicularis, Trichuris trichiura, and Ancylostoma duodenale. From 5 areas of IPAL Terpadu Kota Malang used in this research only in Bareng area it is not found while in four others found 3 kinds of worm egg they are A. lumbricoides, E. vermicularis, and T. trichiura. Egg prevalence of each kind of parasitic worm species on the elementary shool students lives in IPAL Kota Malang area are A. lumbricoides is 65.22%, E. vermicularis is 21.47%, T. trichiura is 11.59%, and A. duodenale is 1.45%. Egg prevalence os each parasitic worm species in IPAL Terpadu Kota Malang are A. lumbricoides is 88.40%, E.vermicularis is 10.15%, T. trichiura is 1.45%, and A. duodenale is 0%. In general is a relationship between the number of parasitic worm egg on the hans and nails of elementary school students lives in IPAL area and worm egg in the water of housing dump in IPAL Terpadu Kota Malang.
TINGKAT PLOIDI DAN TIPE REPRODUKSI Dryopteris sparsa DI HUTAN WISATA CANGAR KOTATIF BATU JAWA TIMUR Siti Zubaidah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/380

Abstract

Study of ploidy level and reproduction type of the fern species Dryopteris sparsa growing in Hutan Wisata Cangar (HWC) Kotatif Batu Jawa Timur and their relationship to the variation of leaf form and size was carried out. Ploidy level was determined based on somatic chromosome number counts by using standard squash methods. Reproduction type was examined by a spore count methods. The morphological characters that examined were the blade length, ratio of basal pinna length to blade length, ratio of stipe length to leaf length and ratio of basal basiscopic pinnule length to basal pinna length. We found that D. sparsa have two forms of ploidy levelreproduction type, there were sexual diploid (2n = 58) and sexual tetraploid (2n = 164). They could be differentiated by the variation of leaf form and size.
KONSTRUKSI MUTASI DAERAH RESISTEN RIFAMPIN (GEN rpoB) DARI Mycobacterium leprae PADA PENDERITA LEPRA DI SURABAYA MELALUI ANALISIS GENOM HASIL PCR E Bimo Aksono H
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/381

Abstract

Rifampin is a key component in the chemoterapeutic regimens used to combat both leprosy and tuberculosis. Owing to exquisite rifampin susceptibility of Mycobacterium leprae, this drug is the backbone of the multidrug therapy currently recommended by WHO for the treatment of leprosy. Resistant mutant are known to arise in leprosy patients receiving rifampin (RIF) monotherapy. The aim of this study was to elucidation of the sequence of the M. leprae rpoB gene permitted identification of mutations associated with rifampin resistance of leprosy patients in Surabaya by genome analysis. M. leprae was detected by nested PCR. In brief, PCR was run with the sense primer rpoBF and anti sense primer rpoBR for 45 cycles. Amplified DNA was analyzed by 3% agarose electrophoresis and the 342 base pairs product was visualized by UV fluorescence after staining with ethidium bromide. PCR product will be purified by phenolchloroform methods and then sequencing directly by ABI PRISM 310. After that sequence data from samples will be analyzed by Genetic Mac ver. 8.0, and comparing with reference data from Gen bank. The result show that only six of 10 samples could be analyzed construct of mutations by Genetic Mac ver 8.0. They have construct no mutation or 100% homology with reference (Z14314 or GI:44382).
KONFIRMASI SPESIFITAS GAD65 TERHADAP ANTI-GAD65 PADA TIKUS DM DAN PASIEN DM TIPE 1 Aulanni’am Aulanni’am; Djoko Wahono Soeatmadji; Sutiman B Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/382

Abstract

The use of glutamic acid decarboxylase (GAD65) from bovine brain has been studied to obtain basic knowledge and diagnosis and prediction of Type 1 Diabetes Mellitus (DM) patients. The importance of GAD65 in DM diagnosis based on its patogenesis. One of the autoimmune marker that can be used to detect beta-pancreas destruction in Diabetes Type I is the antibody to glutamic acid decarboxylase (GAD65). Most of the pre-diabetic patients indicate the reactive autoantibody to GAD65. For early detection of anti-GAD65 in the serum of the patient, human recombinat GAD65 has been succeed to be used. However this is not economical, therefore, it is necessary to find the alternative source of cheaper GAD65. The aim of this research is to develop an early detection kit of Type 1 DM based on antibody-GAD65, since the longest patient suffering from DM has higher probability to be complicated, especially for uncured patients. The anti- GAD65 antibodies induced by anti-GAD65 synthetized and labelled by alkaline phosphatase can be used as reagent detection early DM patients. The ten patients of DM as samples (positive of anti-GAD65) and five rats of DM were positive with western blott technique using reagents as result of this research. It can be concluded, GAD65 enzyme isolated from bovine brain induced anti-GAD65 production and have possibilities to be packaged in a diagnostic kit for patient pre DM.
PENGARUH PERASAN UMBI AKAR LOBAK (Rhapanus sativus L) TERHADAP PENINGKATAN KADAR PROLAKTIN DALAM DARAH MENCIT (Mus musculus) BETINA Mas Loegito; Paulus Liben; Thin Soedarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/383

Abstract

The objective of this research was to prove that the administration of radish tuber extract (Rhapanus sativus L) to female mice (Mus musculus) might increase blood prolactin level. This was an experimental research using complete randomized design by involving three treatment groups and three control group, each with five replications. Materials consisted of radish tuber extract and 30 female mice of 8 weeks old divided into 6 groups, i.e., control groups of C1, C2, and C3, and treatment groups of T1, T2, and T3. C1, C2, and C3 groups were given water of 1 × 0.5 cc/day per oral, 2 × 0.5 cc/day, and 3 × 0.5 cc/day respectively. Treatment groups of T1, T2, and T3 were given radish tuber extract of respectively 1 × 0.5 cc/day per oral, 2 × 0.5 cc/day, and 1 × 0.5 cc/day. Treatment was carried out for 21 days. Blood was subsequently taken from those rats for blood prolactin level. Data were analyzed using Anove test. If the results indicated significant difference of 95% (p = 0.05), the analysis was continued with LSD. The conclusions of this experiment is the administration of radish tuber extract per oral may result in the increase of blood prolactin level.
SIFAT KUALITATIF DAN KUANTITATIF KUSKUS DI PULAU MOOR KABUPATEN NABIRE PAPUA Supriyantono Supriyantono; M Jen Wajo AL; Killian SD; Rumetor Rumetor; Abdillah D Sawen
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/384

Abstract

Reserch on cuscus diversity at Moor island was carried out on November to December 2002. The aim of the research was to know qualitative and quntitative traits, feed and mating behaviour, anatomy of digestion tract and anatomy reproduction organ. Method of case study was used in the research. We found two species of cuscus at Moor island which were Phalanger orientalis and Spilocuscus maculatus. Generally, S. maculatus is bigger than P. orientalis. Both of them can be distinguished easily. Digestion tract of S. maculatus is better than P. orientalis. The form of reproduction organ of those cuscus is very specific and no differ each other.
PENGARUH WAKTU INSEMINASI TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA PASCAINSEMINASI PADA KAMBING Indah Norma Triana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/385

Abstract

The purpose of this research was to know the effect of insemination time on motility and viability of spermatozoa in egg yolk tris diluter post insemination of goat. In this research 20 female goats was used and divided into two groups and synchronized with PGF2 alfa. If sign of oestrus appeared, then goats in group I, were inseminated with semen from buck diluted with egg yolk tris at the beginning of oestrus and group II inseminated with semen from buck diluted with egg yolk tris at the mid oestrus. Semen was collected from cervical canal of goats at one, two, 3, 6, or 24 hours after insemination for evaluating its motility and viability. Implication of this research is indicate that artificial insemination can be conducted in the early also the mid oestrus of goat.
HUBUNGAN KADAR MDA SPERMA DENGAN INTEGRITAS MEMBRAN SPERMATOZOA TIKUS (Rattus norvegicus) SETELAH PEMAPARAN 2–METHOXYETHANOL Alfiah Hayati; Soesanto Mangkoewidjojo; Aucky Hinting; Sukarti Moeljopawiro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/386

Abstract

In the body, 2-methoxyethanol compound may be converted to MAA. MAA is a strong oxidant and may cause oxidation stress in spermatozoa. Oxidation stress is a disturbance on phosphorilation that increases ROS concentration, and it produces lipid peroxide in spermatozoa membrane resulted in high MDA concentration. One of indicator of spermatozoa membrane disturbances is a lack of spermatozoa membrane integrity. The main purpose of this research was to determine the relationship between MDA concentration in sperm and membrane integrity of spermatozoa in rats. The animal of treated groups (n = 40) were divide into 8 groups of 5 each. The rats were given subcutaneous injection with 0,2 ml of 200 mg/kg/day for 1 day (P1), 3 days (P2), 6 days/week (P3), and 12 days/two weeks (P4), respectively the control group was injected with physiological saline of the same volume. The concentration of MDA was measured by spectrophotometer and observing membrane integrity used HOS method to watch the spermatozoa response on hypoosmotic condition. The results of the research indicated that 2-ME caused the increasing in sperm MDA concentration and the decrease of spermatozoa membrane integrity. There was negative correlations between MDA concentration and spermatozoa membrane integrity.
PENGARUH ANTIMITOSIS EKSTRAK Achyranthes Aspera Linn PADA PEMBELAHAN SEL EMBRIO (CLEAVAGE) Wurlina Wurlina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/387

Abstract

To study the antimitotic effect of an extract of the leaf Achyranthes asapera Linn on embryonal cleavage rats (Rattus norwegicus). Rats ova were fertilized in vitro, and thes suitable zygote and embryo were selected to use as samples, which were divided into 5 groups. Each group comprised 80 ova/embryo. Those groups were: group 1. ova for fertilization, group 2. zygote, group 3. 2 cell embryo, group 4. 4 cell embryo and group 5. 8 cell embryo. Each group was divided into 4 treatmens, each of which consisted of 20 ova/ embryos. The treatments were as follows: control treatment receiving TCM 199 media, and treatment 1, 2 and 3 receiving Achyranthes aspera Linn alkaloid of 20, 30, and 40 ppm respectively. Observation to embryonal cleavage and development was out 12 and 24 hours after treatment. Concluded from the results that the administration of Achyranthes aspera Linn as much as 30 ppm in vitro could 1) inhibits fertilization zygote and 2, 4, and 8 cell embryonal cleavage, growth and development 2) induced zygote and embryonal membrane recruitment and blastomere degeneration and 3) inhibits zygote and embryonal mitotic cleavage at metaphase stage.

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